男性雄激素源性脱发患者毛囊干细胞增殖和凋亡的初步研究(1)
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[摘要]目的：了解男性雄激素源性脱发(androgenetic alopecia，AGA)患者毛囊干细胞增殖和凋亡情况。方法：对来自15例男性AGA患者和3例正常人的头皮，采用双重免疫荧光的的方法检测毛囊干细胞增殖和凋亡。结果：CK15标记的毛囊干细胞中增殖细胞核抗原(proliferating cell nuclear antigen，PCNA)阳性细胞率由高到低依次为：正常对照组、患者枕部、脱发过渡区和脱发区，除脱发过渡区与脱发区间比较无显著性差异(P>0.05)外，其它各组间比较，均有显著性差异(P<0.05)。各组毛囊中CK15阳性的细胞中均无TUNEL阳性细胞，TUNEL阳性细胞主要分布于内毛根鞘，总的TUNEL阳性细胞率在各组间无明显差异(P均>0.05)。结论：毛囊干细胞增殖活性下降可能参与男性AGA的脱发过程。
[关键词]雄激素源性脱发;毛囊干细胞;增殖;凋亡
[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455(2011)03-0403-03
The proliferation and apoptosis of hair follicle stem cells in hair follicle in male patients with androgentic alopecia
DONG Jia-hui,WAN Miao-jian,FENG Zhiying,LI Ying,LI Xiaoxin,GONG Zijian,LAI Wei,JI Bing
(Department of Dermatology,The 3rd Affiliated Hospital of Sun Yat-San University,Guangzhou 510630,Guangdong,China)
Abstract: Objective To investigate the proliferation and apoptosis of hair follicle stem cells in male patients with AGA. Methods The follicle from different scalp areas (occipital of non-hair loss, hair loss transition and hair loss zone ) of 15 AGA patients and 3 normal scalp. Using double immunofluorescence method to study the proliferation and apoptosis of hair follicle stem cell in scalp different regions. Results In hair follicle bulge area of normal group and patients' occipital area,there is no significant difference between the percentage of PCNA-positive cells in CK15 positive cells (P>0.05)，they both are higher than that of the hair loss area and the hair loss transition area(P<0.05). There are no TUNEL-positive cells in the CK15-positive cells in each group, TUNEL-positive cells are mainly distributed in the inner hair follicle root sheath, The rate of TUNEL-positive cells is no significant difference among the groups (P>0.05). Conclusion Decrease of the proliferation activity of the hair follicle stem cells may be involved in the pathogenesis of male patients with AGA.
Key words: AGA; hair follicle stem cells; proliferation; apoptosis
AGA是一种常见的进行性脱发性疾病，不仅影响患者容貌，而且给患者心理和社交造成一定的影响[1]。目前，AGA发病的分子机理尚未完全阐明。
毛囊干细胞定居于毛囊外毛根鞘的隆突部位[2] ......
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